Pawan Kumar*, Mathews Michael
Type 2 Diabetes Mellitus (T2DM) has developed into a significant cause of death and morbidity for millions of people worldwide. Antidiabetic drug toxicity concerns have shifted focus towards herbal remedies, which are both cost- was an effective and safe. Therefore, the objective of our study was to investigate the antidiabetic, antihyperlipidemic and antioxidant activities of a novel Polyherbal Formulation (PHF). To prepare the PHF, aqueous extracts of various plant parts namely Commiphora mukul, A$zadirachta indica antioxidant activities of a novel Polyherbal Formulation (PHF). The efficacy of the PHF was studied using various parts Gaertn, 2cimum sanctum / and Trigonella foenum-graecum / Curcuma longa /, ( mblica officinalis Gaertn, Gymnema sylvestre Retz, Momordica Momordica charantia /, Garcinia cambogia , High Fat Diet (HFD) and low dose streptozotocin (STZ-35 mg/kg) induced in rats. An Oral Glucose Tolerance Test (OGTT) was also performed in diabetic rats for assessment of pancreatic β-cell function. On other hand, the group of diabetic rat were fed with the dose is 50, 100 and 200 mg/kg of PHF along with the normal, diabetic and positive control (glibenclamide-0.6 mg/kg) group for 28 days. In addition, the biochemical parameters such aspartate many AminoTransferase (AST), Alkaline Phosphatase (ALP), alanine aminotransferase, and blood glucose and lipid were profile were estimated by auto-analyzer. Insulin, leptin and tumor necrosis factor alpha (TNF-α) are also examined by ELISA kit. The administration of 50, 100 and 200 mg/kg doses of PHF and glibenclamide (0.6 mg/kg), significantly (p<0.05) lower blood glucose level, Low-Density Lipoprotein Cholesterol (LDL-C), Very Low-Density Lipoprotein Cholesterol (VLDL-C), triglyceride, total cholesterol, glycated hemoglobin (HbA1c), TNF- α, leptin, ALT, AST, ALP, creatinine and urea as compared to diabetic control group. The levels of High-Density Lipoprotein Cholesterol (HDL-C), total protein and insulin were found to be significantly increase the compared with diabetic control group. Furthermore, the in vivo antioxidant enzymes such as CAT, SOD, GSH, GPx, and TBARS in liver and kidney tissues showed in significant (p<0.05) recovery in diabetic rats treated with the PHF.